What is the importance of rapid detection techniques for food microorganisms? Explain immunological methods of rapid detection.
What is the importance of rapid detection techniques for food microorganisms? Explain immunological methods of rapid detection.-Foodborne disease incidence has
increased over time, resulting in a major public health problem worldwide.
Foodborne pathogens can be found in a variety of foods, and it is critical to
detect them in order to provide a safe food supply and prevent foodborne
diseases. Traditional methods for detecting foodborne pathogens are time
consuming and labour intensive. As a result, many methods for rapid detection
of foodborne pathogens have been developed, as it is required in many food
analyses. Rapid detection methods are classified as nucleic acid-based, biosensor-based,
or immunological-based. The principles and application of recent rapid methods
for the detection of foodborne bacterial pathogens are highlighted in this
review.
What is the importance of rapid detection techniques for food microorganisms? Explain immunological methods of rapid detection.-Simple polymerase chain
reaction (PCR), multiplex PCR, real-time PCR, nucleic acid sequence-based
amplification (NASBA), loop-mediated isothermal amplification (LAMP), and
oligonucleotide DNA microarray are examples of nucleic acid-based detection
methods; optical, electrochemical, and mass-based biosensors are examples of
biosensor-based methods; enzyme-linked immunosorbent assay (ELISA) and lateral
flow immunoassay Rapid detection methods, in general, are time-efficient,
sensitive, specific, and labor-saving. Rapid detection methods are critical in
the prevention and treatment of foodborne diseases.
What is the importance of rapid detection techniques for food microorganisms? Explain immunological methods of rapid detection.-Unmunochemical methods based on the principle of antibody and
antigen interaction can now be used to detect microbial contamination in foods.
Radioimmunoassay (RIA), enzyme linked immunosorbent assay (ELISA), affinity
chromatography, immunofluorescence, and agglutination are some of the assay
methods used.
0 comments:
Note: Only a member of this blog may post a comment.